The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding.Hunong 5 has high commercial value due to its high polysaccharide and triterpene content.This is the first report of using a DNA pooling method to Collections develop a stable sequence characterized amplified region (SCAR) marker for rapid identification of the G.
lucidum Hunong 5 cultivar.The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat (ISSR) fragment (882 bp) from G.lucidum Hunong 5 cultivar.
A stable SCAR primer pair GLH5F/GLH5R were obtained to identify Cover Support the cultivar and the SCAR marker is a DNA fragment of 773 bp.